Real-time evidence of in vivo leukocyte trafficking in human skin by reflectance confocal microscopy.

(Fig 2a) or by MSP-PCR (Fig 2b). Our results suggest little or no involvement of genetic or epigenetic alterations of CDKN2A in nevus etiology, and thus support the data of others who have failed to detect CDKN2A mutation (Healy et al, 1996b) or methylation (Gonzalgo et al, 1997) in various types of nevi; however, our ®ndings do not rule out mutations in noncoding regions nor factors other than methylation affecting expression of this gene. Alternatively, variations in noncoding regions of CDKN2A or in a gene adjacent to CDKN2A may account for the variance in nevus count we have shown to be linked to this region (Zhu et al, 1999).